cell lines cell line source s hek293t Search Results


cell lines cell line source s hek 293t crl 3216tmatcc  (ATCC)
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cell lines cell line source s hek293t  (TaKaRa)
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TaKaRa cell lines cell line source s hek293t
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dsmz crl 1573 hek293t gifted crl 3216 hct  (DSMZ)
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DSMZ dsmz crl 1573 hek293t gifted crl 3216 hct
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hek293t system hek293t  (ATCC)
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ATCC hek293t system hek293t
Genes cloned for overexpression in a <t> HEK293T system </t> Single cells identified as astrocytes on the basis of established marker genes ( 19 ) were analyzed to identify transcripts fulfilling the following criteria. First, transcripts had to be present in ≥90% of identified astrocytes. Second, transcripts had to encode proteins containing a predicted transmembrane region. Third, only proteins localizing to the plasma membrane were considered. Only proteins fulfilling these criteria could realistically be used in the immunoisolation of live (non-fixed) astrocytes. This table lists identified transcripts fulfilling these criteria and the corresponding PubMed accession numbers of the transcripts used to design subsequent cloning steps. Essentially, PCR primers were designed to flank ORFs of genes of interest (capital letters) as well as to incorporate unique restriction sites on both ends (lowercase letters). Sequences were then amplified by PCR before ligation into the pCAGIG plasmid. Plasmids were verified by sequencing and checked against the deposited amino acid sequence for the respective proteins. Single-nucleotide polymorphisms that did not affect the amino acid sequence were tolerated. In addition to encoding genes of interest, these constructs also encoded cytosolic GFP to act as a marker for cell transfection.
Hek293t System Hek293t, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell lines cell line source s hek293t  (ATCC)
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ATCC cell lines cell line source s hek293t
Genes cloned for overexpression in a <t> HEK293T system </t> Single cells identified as astrocytes on the basis of established marker genes ( 19 ) were analyzed to identify transcripts fulfilling the following criteria. First, transcripts had to be present in ≥90% of identified astrocytes. Second, transcripts had to encode proteins containing a predicted transmembrane region. Third, only proteins localizing to the plasma membrane were considered. Only proteins fulfilling these criteria could realistically be used in the immunoisolation of live (non-fixed) astrocytes. This table lists identified transcripts fulfilling these criteria and the corresponding PubMed accession numbers of the transcripts used to design subsequent cloning steps. Essentially, PCR primers were designed to flank ORFs of genes of interest (capital letters) as well as to incorporate unique restriction sites on both ends (lowercase letters). Sequences were then amplified by PCR before ligation into the pCAGIG plasmid. Plasmids were verified by sequencing and checked against the deposited amino acid sequence for the respective proteins. Single-nucleotide polymorphisms that did not affect the amino acid sequence were tolerated. In addition to encoding genes of interest, these constructs also encoded cytosolic GFP to act as a marker for cell transfection.
Cell Lines Cell Line Source S Hek293t, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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source hek293t cell line dsmz acc 635  (DSMZ)
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DSMZ source hek293t cell line dsmz acc 635
Genes cloned for overexpression in a <t> HEK293T system </t> Single cells identified as astrocytes on the basis of established marker genes ( 19 ) were analyzed to identify transcripts fulfilling the following criteria. First, transcripts had to be present in ≥90% of identified astrocytes. Second, transcripts had to encode proteins containing a predicted transmembrane region. Third, only proteins localizing to the plasma membrane were considered. Only proteins fulfilling these criteria could realistically be used in the immunoisolation of live (non-fixed) astrocytes. This table lists identified transcripts fulfilling these criteria and the corresponding PubMed accession numbers of the transcripts used to design subsequent cloning steps. Essentially, PCR primers were designed to flank ORFs of genes of interest (capital letters) as well as to incorporate unique restriction sites on both ends (lowercase letters). Sequences were then amplified by PCR before ligation into the pCAGIG plasmid. Plasmids were verified by sequencing and checked against the deposited amino acid sequence for the respective proteins. Single-nucleotide polymorphisms that did not affect the amino acid sequence were tolerated. In addition to encoding genes of interest, these constructs also encoded cytosolic GFP to act as a marker for cell transfection.
Source Hek293t Cell Line Dsmz Acc 635, supplied by DSMZ, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hek293t cells  (ATCC)
99
ATCC hek293t cells
Genes cloned for overexpression in a <t> HEK293T system </t> Single cells identified as astrocytes on the basis of established marker genes ( 19 ) were analyzed to identify transcripts fulfilling the following criteria. First, transcripts had to be present in ≥90% of identified astrocytes. Second, transcripts had to encode proteins containing a predicted transmembrane region. Third, only proteins localizing to the plasma membrane were considered. Only proteins fulfilling these criteria could realistically be used in the immunoisolation of live (non-fixed) astrocytes. This table lists identified transcripts fulfilling these criteria and the corresponding PubMed accession numbers of the transcripts used to design subsequent cloning steps. Essentially, PCR primers were designed to flank ORFs of genes of interest (capital letters) as well as to incorporate unique restriction sites on both ends (lowercase letters). Sequences were then amplified by PCR before ligation into the pCAGIG plasmid. Plasmids were verified by sequencing and checked against the deposited amino acid sequence for the respective proteins. Single-nucleotide polymorphisms that did not affect the amino acid sequence were tolerated. In addition to encoding genes of interest, these constructs also encoded cytosolic GFP to act as a marker for cell transfection.
Hek293t Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hek 293t cells  (InvivoGen)
92
InvivoGen hek 293t cells
Genes cloned for overexpression in a <t> HEK293T system </t> Single cells identified as astrocytes on the basis of established marker genes ( 19 ) were analyzed to identify transcripts fulfilling the following criteria. First, transcripts had to be present in ≥90% of identified astrocytes. Second, transcripts had to encode proteins containing a predicted transmembrane region. Third, only proteins localizing to the plasma membrane were considered. Only proteins fulfilling these criteria could realistically be used in the immunoisolation of live (non-fixed) astrocytes. This table lists identified transcripts fulfilling these criteria and the corresponding PubMed accession numbers of the transcripts used to design subsequent cloning steps. Essentially, PCR primers were designed to flank ORFs of genes of interest (capital letters) as well as to incorporate unique restriction sites on both ends (lowercase letters). Sequences were then amplified by PCR before ligation into the pCAGIG plasmid. Plasmids were verified by sequencing and checked against the deposited amino acid sequence for the respective proteins. Single-nucleotide polymorphisms that did not affect the amino acid sequence were tolerated. In addition to encoding genes of interest, these constructs also encoded cytosolic GFP to act as a marker for cell transfection.
Hek 293t Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lenti x hek293t cells  (TaKaRa)
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TaKaRa lenti x hek293t cells
Genes cloned for overexpression in a <t> HEK293T system </t> Single cells identified as astrocytes on the basis of established marker genes ( 19 ) were analyzed to identify transcripts fulfilling the following criteria. First, transcripts had to be present in ≥90% of identified astrocytes. Second, transcripts had to encode proteins containing a predicted transmembrane region. Third, only proteins localizing to the plasma membrane were considered. Only proteins fulfilling these criteria could realistically be used in the immunoisolation of live (non-fixed) astrocytes. This table lists identified transcripts fulfilling these criteria and the corresponding PubMed accession numbers of the transcripts used to design subsequent cloning steps. Essentially, PCR primers were designed to flank ORFs of genes of interest (capital letters) as well as to incorporate unique restriction sites on both ends (lowercase letters). Sequences were then amplified by PCR before ligation into the pCAGIG plasmid. Plasmids were verified by sequencing and checked against the deposited amino acid sequence for the respective proteins. Single-nucleotide polymorphisms that did not affect the amino acid sequence were tolerated. In addition to encoding genes of interest, these constructs also encoded cytosolic GFP to act as a marker for cell transfection.
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hek293t cells  (System Biosciences Inc)
90
System Biosciences Inc hek293t cells
Genes cloned for overexpression in a <t> HEK293T system </t> Single cells identified as astrocytes on the basis of established marker genes ( 19 ) were analyzed to identify transcripts fulfilling the following criteria. First, transcripts had to be present in ≥90% of identified astrocytes. Second, transcripts had to encode proteins containing a predicted transmembrane region. Third, only proteins localizing to the plasma membrane were considered. Only proteins fulfilling these criteria could realistically be used in the immunoisolation of live (non-fixed) astrocytes. This table lists identified transcripts fulfilling these criteria and the corresponding PubMed accession numbers of the transcripts used to design subsequent cloning steps. Essentially, PCR primers were designed to flank ORFs of genes of interest (capital letters) as well as to incorporate unique restriction sites on both ends (lowercase letters). Sequences were then amplified by PCR before ligation into the pCAGIG plasmid. Plasmids were verified by sequencing and checked against the deposited amino acid sequence for the respective proteins. Single-nucleotide polymorphisms that did not affect the amino acid sequence were tolerated. In addition to encoding genes of interest, these constructs also encoded cytosolic GFP to act as a marker for cell transfection.
Hek293t Cells, supplied by System Biosciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hek 293t cells  (Thermo Fisher)
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Thermo Fisher hek 293t cells
Genes cloned for overexpression in a <t> HEK293T system </t> Single cells identified as astrocytes on the basis of established marker genes ( 19 ) were analyzed to identify transcripts fulfilling the following criteria. First, transcripts had to be present in ≥90% of identified astrocytes. Second, transcripts had to encode proteins containing a predicted transmembrane region. Third, only proteins localizing to the plasma membrane were considered. Only proteins fulfilling these criteria could realistically be used in the immunoisolation of live (non-fixed) astrocytes. This table lists identified transcripts fulfilling these criteria and the corresponding PubMed accession numbers of the transcripts used to design subsequent cloning steps. Essentially, PCR primers were designed to flank ORFs of genes of interest (capital letters) as well as to incorporate unique restriction sites on both ends (lowercase letters). Sequences were then amplified by PCR before ligation into the pCAGIG plasmid. Plasmids were verified by sequencing and checked against the deposited amino acid sequence for the respective proteins. Single-nucleotide polymorphisms that did not affect the amino acid sequence were tolerated. In addition to encoding genes of interest, these constructs also encoded cytosolic GFP to act as a marker for cell transfection.
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Image Search Results


Genes cloned for overexpression in a HEK293T system Single cells identified as astrocytes on the basis of established marker genes ( 19 ) were analyzed to identify transcripts fulfilling the following criteria. First, transcripts had to be present in ≥90% of identified astrocytes. Second, transcripts had to encode proteins containing a predicted transmembrane region. Third, only proteins localizing to the plasma membrane were considered. Only proteins fulfilling these criteria could realistically be used in the immunoisolation of live (non-fixed) astrocytes. This table lists identified transcripts fulfilling these criteria and the corresponding PubMed accession numbers of the transcripts used to design subsequent cloning steps. Essentially, PCR primers were designed to flank ORFs of genes of interest (capital letters) as well as to incorporate unique restriction sites on both ends (lowercase letters). Sequences were then amplified by PCR before ligation into the pCAGIG plasmid. Plasmids were verified by sequencing and checked against the deposited amino acid sequence for the respective proteins. Single-nucleotide polymorphisms that did not affect the amino acid sequence were tolerated. In addition to encoding genes of interest, these constructs also encoded cytosolic GFP to act as a marker for cell transfection.

Journal: The Journal of Biological Chemistry

Article Title: An immunoaffinity-based method for isolating ultrapure adult astrocytes based on ATP1B2 targeting by the ACSA-2 antibody

doi: 10.1074/jbc.M116.765313

Figure Lengend Snippet: Genes cloned for overexpression in a HEK293T system Single cells identified as astrocytes on the basis of established marker genes ( 19 ) were analyzed to identify transcripts fulfilling the following criteria. First, transcripts had to be present in ≥90% of identified astrocytes. Second, transcripts had to encode proteins containing a predicted transmembrane region. Third, only proteins localizing to the plasma membrane were considered. Only proteins fulfilling these criteria could realistically be used in the immunoisolation of live (non-fixed) astrocytes. This table lists identified transcripts fulfilling these criteria and the corresponding PubMed accession numbers of the transcripts used to design subsequent cloning steps. Essentially, PCR primers were designed to flank ORFs of genes of interest (capital letters) as well as to incorporate unique restriction sites on both ends (lowercase letters). Sequences were then amplified by PCR before ligation into the pCAGIG plasmid. Plasmids were verified by sequencing and checked against the deposited amino acid sequence for the respective proteins. Single-nucleotide polymorphisms that did not affect the amino acid sequence were tolerated. In addition to encoding genes of interest, these constructs also encoded cytosolic GFP to act as a marker for cell transfection.

Article Snippet: Overexpression of ORF-encoding plasmids, ACSA-2 staining, and flow cytometry Heterologous expression of identified genes in a HEK293T system HEK293T (human embryonic kidney) cells (sourced from ATCC) were maintained according to standard protocols ( 49 ) and were mycoplasma-free.

Techniques: Clone Assay, Over Expression, Marker, Clinical Proteomics, Membrane, Cloning, Amplification, Ligation, Plasmid Preparation, Sequencing, Construct, Transfection

Flow cytometry-based experiments identify ATP1B2 as a target for ACSA-2. HEK293T cells (which do not bind ACSA-2 under normal conditions) were transfected with plasmids encoding for proteins identified by our bioinformatic screen (see Table 3). These plasmids also expressed soluble GFP as a marker for successful plasmid transfection. Cells were then stained with an ACSA-2-APC conjugate and analyzed by flow cytometry. From the list of proteins identified in Table 3, only cells expressing ATP1B2 showed strong co-labeling for ACSA-2 and GFP (28.2% of cells). A representative experiment is presented in the figure. This experiment was repeated twice using independent samples (effectively 1 technical replicate per sample) on separate days with the same results. At least 60,000 cells were analyzed per sample. Lines in each plot delineate gates; numbers represent the proportion of cells in each particular gate.

Journal: The Journal of Biological Chemistry

Article Title: An immunoaffinity-based method for isolating ultrapure adult astrocytes based on ATP1B2 targeting by the ACSA-2 antibody

doi: 10.1074/jbc.M116.765313

Figure Lengend Snippet: Flow cytometry-based experiments identify ATP1B2 as a target for ACSA-2. HEK293T cells (which do not bind ACSA-2 under normal conditions) were transfected with plasmids encoding for proteins identified by our bioinformatic screen (see Table 3). These plasmids also expressed soluble GFP as a marker for successful plasmid transfection. Cells were then stained with an ACSA-2-APC conjugate and analyzed by flow cytometry. From the list of proteins identified in Table 3, only cells expressing ATP1B2 showed strong co-labeling for ACSA-2 and GFP (28.2% of cells). A representative experiment is presented in the figure. This experiment was repeated twice using independent samples (effectively 1 technical replicate per sample) on separate days with the same results. At least 60,000 cells were analyzed per sample. Lines in each plot delineate gates; numbers represent the proportion of cells in each particular gate.

Article Snippet: Overexpression of ORF-encoding plasmids, ACSA-2 staining, and flow cytometry Heterologous expression of identified genes in a HEK293T system HEK293T (human embryonic kidney) cells (sourced from ATCC) were maintained according to standard protocols ( 49 ) and were mycoplasma-free.

Techniques: Flow Cytometry, Transfection, Marker, Plasmid Preparation, Staining, Expressing, Labeling